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Comparative study of the functioning and the efficiency of two experimental biogas measuring devices

I. GHARIANI1,2

O. ELASRI3

H. LAICHE3

T. NAJAR1,2

 

1 Animal Production Department, National Agronomic Institute in Tunis, University of Carthage, Tunis, Tunisia

2 Laboratory of Materials Molecules and Applications, Preparatory Institute for Scientific and Technical Studies, University of Carthage, Tunis, Tunisia

3 Biochemistry and biotechnology Laboratory, Mohamed First University, Oujda; Morocco




Abstract – Several wastes can be treated by Anaerobic Digestion (AD) to minimize their adverse effects. Different parameters can affect the wastes valorization such as their origin, as well as the used processes and devices. The purpose of this study is to compare two experimental devices for biogasmeasurement and to select the best for its biogas production and laboratory scale operation. The substrate chosen for this study is glucose with the addition of poultry digestate as an inoculum. The use of erlenmeyer as gasometer filled with wateris often inconvenient for various reasons, namely gas solubility in water and water evaporation and also the difficulty of passing the produced gas especially with low flow rate up to the gasometer. Also, we followed the kinetics of the volume of biogas produced by each device as a function of time and the degradation of Organic Matter (OM). The use of the device with the gasometer formed by reverse graduated burette filled by the barrier solution occur the best results in terms of maximum amount of biogas produced, minimization of the reduction of the pH during the AD and the biodegradation of the OM a recorded an important value which confirms the good performance of this device. These results allow us to conclude that the choice of the second digester for starting experimental AD can minimise gas solibility and promotes the stabilization of optimal conditions of AD.

Keywords: Experimental device, Anaerobic Digestion, Glucose, Biogas

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S-locus diversity of sweet cherry cultivars in Tunisia

T. AZIZI-GANNOUNI1,2*

T. BARRENECHE3

J. QUERO-GARCIA3

J. ANTONIO CAMPOY3

I. OUERGUI4

A. ALBOUCHI2

 Y. AMMARI2

 

1 Faculty of Mathematical, Physical and Natural Sciences of Tunis, Compus El manar 2092, University of El manar, Tunisia.

2 National Researches Institute of Rural Engineering, Water and Forests, Tunis (INRGREF), University of Carthage, Tunisia.

3 INRA Bordeaux UMR1332 fruit biology and pathology (BFP), Villenave d’Ornon Cedex, France.

4 National Institute for agricultural Research (INRA), University of Cartage, Tunsia



Abstract – Sweet cherry (Prunus avium L.) is a species that is characterized by gametophytic self-incompatibility (GSI), which determines the fruit set and, consequently, the tree fruit load. In this study, S-alleles have been identified for nine sweet cherry cultivars in north-western Tunisia. We genotyped 9 cultivars (five introduced unknown cultivars, three international ones and one local ‘Bouargoub’) and we found 2 new Tunisian sweet cherry S-haplotypes (S2 and S10) using polymerase chain reaction (PCR) based on length polymorphism detection of S-RNase and SFB genes. Most introduced sweet cherry cultivars are self-incompatible and cross pollination is necessary to ensure the fruit set. However, the local one “Bouargoub” was considered to be self-compatible. The haplotypes S2and S10 was less frequent, whereas S3 was the most frequent (44.44%) in the nine studied cultivars.

Keywords: Prunus aviumL.polymerase chain reaction; S-RNase; Fruit set

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Copyright

This article is published under license to Journal of New Sciences. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

CC BY 4.0