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Quality mentoring of Greek yoghurt fortified with pomegranate juice and arils (Punica granatum L.) during storage

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S. KHARCHOUFI1*,

M. AHMED ABBAS MAHMOUD2,3,4,

S. LOUPASSAKI5,

M. HAMDI1

 

Laboratory of Microbial Ecology and Technology, National Institute of Applied Sciences and Technology (INSAT), University of Carthage, Centre Urbain Nord, 2 Boulevard de la Terre, B.P. 676, 1080 Tunis, Tunisia

Department of Chemistry and Pharmacy, Emil Fischer Center, Friedrich-Alexander-Universität Erlangen-Nürnberg, Henkestrasse 9, 91054 Erlangen, Germany

3 Departement Sensory Analytics, Fraunhofer Institute for Process Engineering and packaging (IVV), Giggenhauser Str. 35, 85354 Freising, Germany

4 Agricultural biochemistry departement, faculty of agriculture, Ain Shams University, PO Box 68, Hadayek Shobra, 11241 Cairo, Egypt

Department of Food Quality and Chemistry of Natural Products, Mediterranean Agronomic Institute of Chania, Centre International de Hautes Etudes Agronomiques Méditerranéennes, Chania, PO Box 85, 73100 Chania, Crete, Greece

 

 

Abstract – Greek yoghurt is a highly nutritious fermented product characterized by its higher protein, low sodium and low carbohydrate contents, and has gained immense popularity compared to traditional yoghurt. It has a unique semi-solid texture due to manufacturing process. In this study, Greek yoghurt was fortified with pomegranate juice (PJ) and arils (PA) to investigate its thick-matrix effect on the availability of phenolics and dietary antioxidant during 18 days of storage at 4˚C and to get indications about the best treatment that could sustain antioxidants during the storage period. PJ or PA in 20 % were mixed with cultured and dessert Greek yoghurts (CPJ, CPA, DPJ, DPA respectively). The total phenolics (TPC), using Folin-Ciocalteu, and antioxidant activity, using DPPH, FRAP, and luminal-induced chemiluminescence, were tested over four periods of storage (1; 6; 12; 18 days). Organoleptic quality was evaluated as well.
The obtained results indicated that both PJ and PA enhance the TPC and antioxidant activity of both yoghurts, and the cultured yoghurt appeared to be a better matrix to preserve pomegranate’s bioactive molecules.

Keywords: Greek yoghurt; pomegranate; TPC; antioxidant activities; sensorial quality

  1. Introduction 

Pomegranate is a nutritional and healthy fruit that is consumed frequently. It is a desirable choice for patients suffering from high cholesterol levels as well as heart and kidney problems. It also has medicinal values which prevent intestinal disorder and diarrhea. It is known for its high phenolic contents, such as anthocyanins, phytoestrogenic, flavonoids, and tannins (Mousavinejad et al. 2009). In addition to that, pomegranate is an important source of other nutrients including dietary fibre, proteins, oligosaccharides, vitamins, and trace minerals. For this reason, pomegranate extracts are used as bioactive ingredients in several foods products (yoghourt, beef sausage, ice cream) (Sun-Waterhouse 2011; Robert et al. 2010; Çam et al. 2013; El-Nashi et al. 2015). Moreover, level of phenolic compounds is highly affected by harvesting time and ripening degree of fruit (Kelebek et al. 2015; Kelebek et al. 2016).

The diverse range of fruit-based functional food products available in the worldwide markets reflects the confidential using of such products as a delivery method for nutritional active compounds, e.g. flavonols, catechins, anthocyanidins, and isoflavones (Sun-Waterhouse 2011; De Carvalho Alves et al. 2013; Karimi et al. 2016). On the other hand, phenolic antioxidants in some cases tend to bind with the components of food matrix; hence phenolics wouldn't be available to exert its wanted beneficial effect as antiradical, e.g. protein binding to catechins can affect its scavenging ability by lowering the number of free hydroxyl groups (Trigueros et al. 2014). This criterion is less able to affect the antiradical activity of food supported by anthocyanins, where most of the reports indicated that it is the only group that might not be hindered by binding effect of dairy proteins (Trigueros et al. 2014; Karaaslan et al. 2011). Different yoghurt-based functional foods are commercially available under diverse brand names. These products are including probiotic yoghurt drinks and yoghurt rich in fruits polyphenols (Sun-Waterhouse 2011; De Carvalho Alves et al. 2013; Trigueros et al. 2014). In this context, several reports discussed the beneficial addition of pomegranate juice and encapsulated pomegranate extracts to yoghurt (Robert et al. 2010; Trigueros et al. 2014). According to these reports, pomegranate based-yoghurt might have many advantages over plain yoghurt. Thus, the use of pomegranate in yoghurt making would not only improve the product quality but would provide one of the outlets for the fruit during the peak production period when its market rates are sharply lowered (Donkor et al. 2007).

Greek yoghurt is known to be in the top of European strained yoghurt products. It is obtained by removing part of the water from the yoghurt or by adding dried hydrocolloids, e.g. dairy protein, to obtain its unique and desired semi-solid texture (Desai et al. 2013). Moreover, in the US, the production of Greek yoghurt, with about 25% of the total sales, is the largest growing sector among all dairy products (Desai et al. 2013). There are two types of Greek yoghurt available on the market; the cultured yoghurt, that retains viable bacteria after processing, and the dessert yoghurt, where the culture bacteria have died after preparation; however, to the best of our knowledge, the use of pomegranate juice and arils (Punica granatum L.) to produce functional Greek yoghurt has never been discussed. For this reason, 20% of pomegranate juices or arils were added to Greek cultured and dessert yoghurt and the respective extracts were explored for their phenolic content and antioxidant activity during 18 days of storage to investigate the effect of viable bacteria as well as the thick-texture of the yoghurt on the availability of phenolic content and the antiradical activity in the fortified samples, in addition, to evaluate the ability of arils to retain the antiradical effect in comparison with samples based on juice.

 

  1. Materials and methodes
    2.1. Chemicals

Ethyl Acetate, Folin Ciocalteu reagent, sodium carbonate, FeCl3.6H2O, COCl2.6H2O, hydrogen peroxide solution (H2O2) 30%, metaphosphoric acid was obtained from Merck (Germany). Ethanol, methanol (MeOH), 2,2-diphenyl-1-picryhydrazyl radical (DPPH), gallic acid, ascorbic acid, luminol, EDTA, sodium metabisulphate anhydrous, and TPTZ (2,4, 6-tripyridyl-s-triazine) were obtained from Sigma-Aldrich (Germany). Acetone, sodium acetate and ammonium sulphate were purchased from Fluka. Petroleum ether, diethyl ether, n-hexane and hydrochloric acid (37%) were obtained from Riedel-de Haën (Germany).

2.2. Fruit samples preparation

The pomegranate fruits were bought from a local market in Zerkine, Gabes (Tunisia), which is well known of pomegranate fruit production. After throwing of any injured and/or sunburnt fruits, pomegranate fruits were peeled and then any skins around the seeds were removed manually. The arils were separated from the fruits and the juice was extracted via a pilot plant packaged-type press (Tefal Rondo 800 Multifunction). The juice samples were centrifuged (10 min at 3000 rpm at 4 °C). Before the experiments, the pomegranate juice was centrifuged at 10000 rpm for 10 min to remove methanol-insoluble particles. Pomegranate arils were incorporated directly in yoghurt samples.

2.3. Fortification of samples with pomegranate juice or arils

Fresh prepared dessert and cultured yoghurts were purchased from a local Greek market for the experiment. Proportions of 20% yoghurt-flavoured pomegranate juice or arils were prepared in triplicates, with final weight of 100 g. This chosen percentage was selected based on multiple preparation experiments (data not shown). The yoghurt samples were then put inside glass jars covered with plastic cover and conserved in the refrigerator at 4 °C, pomegranate juice were also conserved in plastic tube. Samples of 10 g of yoghurt and 10 ml of juice were then taken in the 1, 6, 12 and 18 day to be analysed.

2.4. Extraction procedure

Extraction of total phenolics was according to Amiot et al. (1986). 10 g of yoghurt enriched with pomegranate arils or juice extract were homogenized with 80% MeOH and 2% metabisulphite for 3 min. After agitation for 20 min, the sample was centrifuged. The procedure was repeated a second time to the pellet and the supernatants were combined. An extraction step with petroleum ether was performed to remove the fat residues (2 50 mL petroleum ether). After evaporating the solvent, the sample extracted solvent was ethyl acetate in the presence of ammonium sulphate (20%), metaphosphoric acid (2%) and methanol was added when emulsion was formed. The ethyl acetate phase, after drying with sodium sulphate, was evaporated until dryness and re-dissolved in 2 mL MeOH and kept in  20 °C due to analysis.

 

2.5. Determination of total phenols content

The total polyphenol content (TPC) of pomegranate juices and the different extracts was determined via the Folin-Ciocalteu method adapted to a microscale (Arnous et al. 2013; Waterman et Mole 1994). Results were expressed as gallic acid equivalents (mg GAE/L).

 

2.6. Determination of total antioxidant activity

2.6.1. DPPH radical scavenging capacityu

The free radical scavenging capacity was determined by the use of stable 2,2-diphenyl-1-picrylhydrazyl radical (DPPH˙) as described by Arnous et al. (2013). An aliquot of 25 μL of sample was added to 975 μL of DPPH solution (60 μM in MeOH) and vortexed. The absorbance was read at = 0 and = 30 min. Ascorbic acid was used as standards and the results were expressed as ascorbic acid equivalent mM/L.

 

2.6.2. Determination of ferric-reducing antioxidant power (FRAP)

For the measurement of the reducing ability of pomegranate-flavoured yoghurt extracts, a protocol based on the ferric reducing antioxidant power (FRAP) assay was applied, as described by Benzie and Strain (1999). A freshly prepared working FRAP solution was prepared by mixing 50 mL of acetate buffer (0.3 M, pH 3.6), 5 mL of 10 mM TPTZ solution in 40 mM HCl, and 5 mL of 20 mM FeCl3- 6H2O solution. Pomegranate-flavoured yoghurt extracts (50 μL) were allowed to react with 1500 μL of the FRAP solution and absorbance was measured at 593 nm ( ) at 0 min after vortexing. Thereafter, the samples were placed at 37°C in a water bath and absorption was measured again after 4 min ( . Ascorbic acid was used as the calibration standard and results were expressed as ascorbic acid equivalent mM/L.

 

2.6.3. Determination of free hydroxyl radical scavenging activity

A chemiluminescence method was used as described by Parejo et al. (2000) and Mahmoud et al. (2013). In this method, H2O2 was used as a radical source which reacts with Co (II) and generates hydroxyl radicals through a Fenton reaction. Then, luminol reacts with a generated hydroxyl radical to form 3-aminophtalate in an excited electronic state which returns to ground state by emission of light. EDTA, as a chelator, is used to decrease the speed of the Fenton reaction. The chemiluminescence intensity of this system reaches a plateau which drops in the presence of an antioxidant. Practically, 1 ml of borate buffer solution (50 mM, pH 9.00) containing CoCl2·6H2O (8.4 mg ml−1) and EDTA (2.63 mg ml−1) was mixed in a test tube with 0.1 ml of luminol solution (0.56 mM in borate buffer 50 mM, pH 9.00), vortexed for 15 s and followed by the addition of 0.025 ml of H2O2 aqueous solution (5.4 mM). After being vortexed for 30 s the mixture was rapidly transferred into a glass cuvette and the CL intensity (blank) was measured when it reached the plateau (Io). For the measurement of the samples an aliquot of 0.025 ml of sample (pure compound or mixtures) was added into the test tube containing the buffer, luminol and H2O2 solutions as above and the CL intensity (I) was recorded at the plateau. The reactions in the glass cuvette were performed under magnetic stirring. The ratio Io/I was calculated and plotted vs. concentration (μmol l−1) of the sample and correlations were established using linear, exponential and polynomial regression analysis. The concentration of sample (IC50), which is required to decrease Io intensity by 50%, was also calculated. For all measurements, a fluorimeter (model 6200, Jenway Ltd., Gransmore, Essex, UK) was used, keeping the lamp off and using only the photomultiplier of the apparatus. Ascorbic acid was used as the calibration standard and results were expressed as ascorbic acid equivalent mM/L.

 

2.7. Sensorial Analysis

Ten trained panelists from the staff members of the, Mediterranean Agronomic Institute, Greece, used a quality rating scorecard for the evaluation of flavor (50 points) body and texture (40 points) and appearance (10 points). Samples were coded differently and presented randomly.

 

2.8. Statistical analysis

Data were presented as the mean ± standard deviations (n=3).Experimental data were analysed with the SPSS 20®programme, performing one-way analysis of variance (ANOVA), and the DUNCAN test was used (a p-value of 0.05 was considered significant).

 

  1. Results and discussion
    3.1. Total phenol content

Based on the present results of TPC (Table 1), pomegranate juice showed a high phenolic content. In fact, pomegranate juice (PJ) is well known as a highly rich source of anthocyanidins, e.g. cyanidin and pelargodinin, and other phenolics, e.g. gallic acid (Trigueros et al. 2014). On the other hand, the plain cultured and dessert yoghurts presented low phenolic content. These results are in agreement with the findings of El-Said et al. (2014) and Trigueros et al. ( 2014)

A significant decline in TPC in all samples was noticed during the 18 days of storage at 4° C. Such a phenomenon was also observed by Varela-Santos et al. (2012). In fact, the gel matrix of yoghurt might protect the phenolics during storage to some extent; however, the declination is thought to be triggered as a result of the degradation of phenolic compounds, mainly anthocyanins, which is influenced by the contact with oxygen (Pérez-Vicente et al. 2004; Trigueros et al. 2014; Mang et al. 2015).

Although, the TPC decreased during the 18 days of storage, a considerable amount was remained in the fortified yoghurt with PJ and PA. This could be attributed to anthocyanins, which are the most stable among all phenolics in yoghurt matrix (Trigueros et al. 2014).

The results showed that PJ appears to be more efficient in increasing the TPC. Furthermore, Palma et al. (2015) found that ready to eat pomegranate arils lose its TPC during 10 days of cold storage at 5°C under modified atmosphere packaging. These results are in consistent with the later finding. Indeed, commercial pomegranate usually has more antioxidant power as it contains peels and rind constituent.

During the storage time, the cultured yoghurt samples exhibit higher total phenol content than the dessert yoghurt ones, including those fortified with PA and PJ. The findings are in line with those found by El-Said et al. (2014) and Sun‐Waterhouse (2011) who reported that bacteria transform polyphenolic compounds in health promoting extract like berry or pomegranate peel extract into smaller unites that are more extractable or stable. As bacterial culture could affect the anthocyanin content during storage which is probably associated with the production of antimicrobial compounds by the starter culture (Trigueros et al. 2014), the convenient culture for the production of yoghurt should be selected in order to guarantee a better stability of anthocyanins (Ścibisz et al. 2012) .

 

 

Table 1. Effect of storage time and different treatments on total phenol content of permanganate juice and Greek yoghurts

 

Sample

Total phenol content (gallic acid equivalent mg/L)

Storage time (day)

1

6

12

18

Cultured yoghurt

116.00±0.78aD

112.34±1.54aC

110.16±0.38bB

104.15±0.65bA

Dessert yoghurt

116.225±0.77aD

111.28±0.5aC

99.38±0.49aB

95.28±0.5aA

PJ

915.39±0.58eD

853.9±1.91fC

764.50±1.27fA

785.11±2.56gB

DYPJ

277.53±1.56dD

211.77±1.1dC

176.86±1.47dB

144.39±0.61dA

CYPJ

279.39±0.5dD

250.21±0.76eC

208.25±0.36eB

198.66±0.32fA

DYPA

195,37±1.61bC

162.39±0.5bB

154.69±0.9cB

135.83±0.75cA

CYPA

209.56±1.11cC

195.82±1.04cC

174.19±1.7dB

166.8±1.13eA

Each value is expressed as mean SD (n=3) ± standard error. Means with different capital letter within a row and uppercase letter within line are significantly different (P< 0.05) according to Duncan’s multiple range test.

 

3.2. Antioxidant activity

The antioxidant activity of PJ, cultured yoghurt and dessert yoghurt mixed with PJ and PA were analysed during the 18 days of storage at 4 °C by mean of DPPH, FRAP and chemilimunescence tests (Tables 2-4). Several tests were used because the antioxidant capacities of samples might be influenced by several factors and could not be fully described by one single method. In addition, most natural antioxidants are multifunctional; therefore, a reliable antioxidant evaluation protocol requires performing different antioxidant activity assessments to consider various mechanisms of antioxidant action (Zhang et al. 2009). 

 

 

Table 2. Effect of storage time and different treatments on the antioxidant activity by mean of DPPH test of permanganate juice and Greek yoghurts

 

Sample

 

DPPH (ascorbic acid equivalent mM/L)

Storage time (day)

1

6

12

18

Cultured yoghurt

2.3±0.21aD

2.07±0.01aC

1.82±0.06aB

1.62±0.04aA

Dessert yoghurt

2.99±0.14bD

2.72±0.05bC

2.61±0.77bB

1.89±0.09aA

PJ

15.65±0.43eD

14.07±0.04eC

13.65±0.41eB

11.50±0.16dA

DYPJ

4.74±0.49cdD

4.38±0.15dC

2.94±0.13cB

2.50±0.16bA

CYPJ

5.07±0.03dD

4.64±0.05dC

3.97±0.08dB

3.05±0.16cA

DYPA

4.39±0.36cD

3.81±0.05cC

2.96±0.65cB

2,51±0.05bA

CYPA

4.94±0.05cdD

4.23±0.05dC

3.82±0.04dB

2.93±0.5cA

Each value is expressed as mean SD (n=3) ± standard error. Means with different capital letter and uppercase letter within a row are significantly different (P< 0.05) according to Duncan’s multiple range test.

 

 

Table 3. Effect of storage time and different treatments on the antioxidant activity by mean of FRAP test of permanganate juice and Greek yoghurts

 

Sample

 

FRAP (ascorbic acid equivalent mM/L)

Storage time (day)

1

6

12

18

Cultured yoghurt

2.61±0.04aD

2.08±0.02aC

1.83±0.05aB

1.28±0.55aA

Dessert yoghurt

2.98±0.05bD

2.56±0.05bC

1.99±0.02aB

1.55±0.55aA

PJ

12.77±0.19eBC

12.13±0.005eB

11.70±0.04dA

11.41±0.13eA

DYPJ

4.72±0.05dD

4.14±0.04cC

3.5±0.055cB

2.93±0.06bA

CYPJ

5.06±0.04cD

4.42±0.02dC

3.81±0.04cB

3.12±0.55cA

DYPA

4.62±0.04cD

4.09±0.01cC

3.42±0.02bB

2.88±0.01bA

CYPA

5.00±0.01dD

4.47±0.1dC

3.82±0.12cB

3.03±0.25cA

Each value is expressed as mean SD (n=3) ± deviation. Means with different lowercase letter within a row and uppercase letter within line are significantly different (P< 0.05) according to Duncan’s multiple range test

 

 

Table 4. Effect of storage time and treatments on the antioxidant activity by mean of Chemiluminescence test (IC50) test of permanganate juice and Greek yoghurts

 

Sample

 

Chemiluminescence (ascorbic acid equivalent mM/L)

Storage time (day)

1

6

12

18

Cultured yoghurt

2.07±0.02aD

1.73±0.06aC

1.40±0.04aB

1.17±0.05aA

Dessert yoghurt

2.22±0.22aD

1.93±0.04aC

1.67±0.09bB

1.48±0.07bA

PJ

11.13±0.04cD

10.49±0.15cC

9.47±0.08eB

9.84±0.06eA

DYPJ

4.67±0.05bD

4.09±0.01bC

2.83±0.035cB

2.42±0.02cA

CYPJ

4.76±0.06bD

4.27±0.07bC

3.32±0.02dB

2.84±0.04dA

DYPA

4.49±0.49bD

4.15±0.15bC

3.02±0.02cB

2.20±0.15cA

CYPA

4.58±0.08bD

4.14±0.15bC

3.37±0.02dB

2.78±0.11dA

Each value is expressed as mean SD (n=3) ± standard deviation. Means with different lowercase letter within a row and uppercase letter within line are significantly different (P< 0.05) according to Duncan’s multiple range test.

 

The antioxidant capacity of PJ and fortified yoghurt samples decreased during the storage time in the different antioxidant capacity measure tests. The antioxidant capacity of both dessert and cultured yoghurts mixed with PJ and PA were even less than the sum of the antioxidant capacity of PJ and plain yoghurt alone. Based on the work of Arts et al. (2009) , this decrease is related to binding effect between polyphenol and dairy protein. For instance, addition of milk to tea reduced the antioxidant capacity of the antioxidants present in tea. The binding proteins and polyphenols reduce antioxidant activity by lowering the number of free hydroxyl (Amiot et al. 1986; Dubeau et al. 2010). Because of the polyphenol-protein complexes, antioxidants bioavailability might be decreased. This phenomenon might also occur in other products containing both polyphenols and proteins.

The interaction, resulting in protein-polyphenol complexes, can be both reversible and irreversible depending on pH, temperature, and protein and flavonoid concentrations. Proteins which contain more proline groups and flavonoids such as epigallocatechin gallate (EGCG) and epicatechin gallate in green tea and gallic acid in black tea are responsible for the depletion of the antioxidant activity (Trigueros et al. 2014). In general, phenolic compounds have more antioxidant activity in acid solution. According to El-Said et al. (2014), at a basic pH, Gallic acid showed less absorbance at 765 nm due to the fact that the pH level can affect and change the ionization state of the phenolic compounds and, therefore, the absorbance maximum. These results could explain the higher antioxidant activities recorded for cultured yoghurt.

The correlation between the total phenol content and the different antioxidant activity parameters, DPPH, FRAP and IC50 (Table 5) was very high for both dessert and cultures yoghurt mixed with pomegranate juice and arils at the end of storage period. These results are in consistent with previous studies which reported that the content of total polyphenols, as determined by the Folin–Ciocalteau reagent, is highly correlated with other antioxidant assays, such as ORAC, DPPH and FRAP ( Ozgen et al. 2008; Madrigal-Carballo et al. 2009).

 

 

Table 5: Correlation matrix between antioxidant capacity and total phenol content of PJ, CPA, CPJ, DPA, and DPJ

 

 

TPC

DPPH

FRAP

IC50

TPC

1

0.992

0.996

0,99

DPPH

0,992

1

0,991

0,998

FRAP

0,996

0,991

1

0,988

IC50

0,99

0,998

0,988

1

 

 

    1. Sensorial analysis

The organoleptic quality of yoghurt enriched with pomegranate juice and arils was assessed during the last day of the trial. The results presented in Table 6 show that both types of yoghurts enriched with pomegranate juice had the best flavor followed by that fortified with arils. Although, the juice has altered the semi-solid texture of Greek yoghurt. From the point of view of appearance, the addition of the juice or arils to both yoghurt was highly appreciated. Thus, the addition of arils and pomegranate juice improved the organoleptic quality of the yoghurt.

 

 

Tableau 6: Organoleptical properties of cultured and dessert yoghurt fortified with pomegranate juice and arils

 

Samples

Flavor

Texture

Appearence

Overall quality

Cultured yoghurt

43,32 ± 1,4 a

38,56 ± 1,58 b

8,52 ± 0,56 a

90,4 ± 1,19 a

Dessert yoghurt

44,18 ± 1,98a

38,15 ± 2,34 b

8,43 ± 1,32 a

90,76 ± 1.89 a

CYPJ

47,56 ± 1,54c

37,23 ± 2,46 a

9,16 ±0,98 b

93,95 ± 1,24 b

CYPA

46,32 ± 2,34b

38,32 ± 1,89 b

9,57 ± 1,14 b

94,21 ± 1,34c

DYPJ

47,13 ± 1,67 c

37,54 ± 3,6 a

9,32 ± 1,04 b

93,99 ±1,57 b

DYPA

45,67 ± 2.03 b

38,13 ± 1,5 b

9,61 ± 1,24 b

93,41 ±1,19 b

Each value is expressed as mean SD (n=3). Means with different lowercase letter within a row are significantly different (P< 0.05).

 

  1. Conclusion

Health-promoting Pomegranate juice appeared to be a promising rich antioxidant product that could improve the quality of dessert and cultured yoghurtsWe conclude that the approaches for adding PJ to cultured and dessert yoghurts are feasible since both matrices could preserve a considerable antioxidant activity and TPC during the time frame of study (18 days), though cultured yoghurt exhibit better interaction. Furthermore, adding PJ to cultured yoghurt is preferred since the seed might cause problems for some consumers especially for elderlies. As a future perspective, more focus on different product processing challenges, microbiological features and functionality advantages are needed to be studied.

 

  1. Acknowledgment

Kharchoufi Samira was supported by a Ph.D. scholarship funded by the Tunisian Ministry of Higher Education and the European Union program of Research and Innovation Project Support “PASRI” (2012).

Mohamed Mahmoud was supported by a Ph.D. scholarship funded by the Egyptian Ministry of Higher Education (MoHE) and the German Academic Exchange Service (DAAD) within the 6th call (2014-2015) of the German-Egyptian Research Long Term Scholarship Program (GERLS; Section 441, No. 91526983).

 

  1. Conflict of interest

The authors declared that there is no conflict of interest.

 

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