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Investigation de la diversité génétique des races Barbe et Arabe Barbe en Tunisie












1 Laboratoire d'Amélioration et de Développement Intégré de la Productivité Animale et des Ressources Alimentaires, Ecole Supérieure d'Agriculture de Mateur, Université de Carthage, Tunisie

2 Fondation Nationale d'Amélioration de la Race Chevaline, Sidi Thabet

3 Laboratoire d'Analyse Génétique Animale, Institut de la Recherche Vétérinaire de Tunisie


Abstract - This study aims to investigate Barb and Arab Barb genetic diversity. In total 200 individuals were analyzed and DNA was amplified using 17 microsatellites. Results showed that a total of 306 alleles from 17 microsatellite loci were detected in 200 horses. The average number of alleles per locus was 8.33 (0.79) and 7.70 (0.613), in the Barb and Arab Barb horses, respectively; whereas the observed and expected heterozygosities per breed were 0.85 (0.03) and 0.86 (0.01). The Barb and Arab-Barb breeds seem to be genetically related.


Keywords: Horses, microsatellites, diversity, Tunisia


Résumé - Dans l’objectif e l’étude de la diversité génétique équine 200 échantillon de sang ont été collectés chez des chevaux de la race Barbe et de la race Arabe Barbe. L’ADN génomique a été amplifié par 17 microsatellites. Les résultats observés ont montré que pour la population Barbe les marqueurs utilisés ont généré une moyenne de 8.33 (0.79) d’allèles. Les taux d’hétérozygotie observé et d’hétérozygotie attendue respectivement de 0.85 (0.03) et 0.86 (0.01). La moyenne des allèles observés chez la population Arabe Barbe est 7.70 (0.613) allèle par locus. Les taux moyens d’hétérozygotie observée d’hétérozygotie attendue respectivement de 0.83 (0.03) et 0.86 (0.01). Les deux populations sont légèrement déficitaires en hétérozygotie. La population totale est moins excédentaire en hétérozygote que les deux populations chacune a part. L’analyse en composante principale, le dendrogramme obtenu ainsi que l’analyse factorielle des correspondances ont montré un chevauchement entre la constitution génétique des individus analysés et ont confirmé la base génétique commune.


Mots clés : chevaux, microsatellites, biodiversité, Tunisie


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Antioxidant capacity and phenolic composition as a function of genetic diversity of wild Tunisian leek (Allium ampeloprasum L.)







Arid Lands Institute, Range Ecology Laboratory, Medenine, Tunisia


Abstract - Wild Allium species with an important use in Tunisia, such as Allium ampeloprasum L. could provide interesting bioactive compounds to current diet and medical. The bioactive compound content and the antioxidants potentialities of this wild species and the influence of the environmental condition on theses characteristic have been scarcely known. In order to further asses this assumption, ten accessions originating from different bioclimatic stages of Tunisia, were compared on the basis of the bioactive compounds content and the antioxidant capacity of the edible parts of wild leek (leaves and bulbs). The total polyphenol (16.64-48.22 mg GEA/g DM), flavonoid (1.01-5.84 mg CE/g DM) and tannin (3.47-7.62 mg CE/g DM) contents and antioxidants activities (DPPH and iron chelating power) were strongly affected by above cited factors. Such variability might be of great importance in terms of the valorizing of these species as a source of naturally products, and the methods for phenolic and antioxidant production.


Keywords: accessions, bioactive compounds, DPPH and iron chelating power, Tunisia


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Potential of Arbuscular Mycorrhization and Fertilizer Application in the Improvement of the Status Nutrition and growth of Jacaranda mimosifolia D.Don Grown under Urban Environment





Institut National Agronomique de Tunisie (INAT), 1082 Tunis - Mahrajène, Tunisie


Abstract - This investigation aims to evaluate the potential of arbuscular mycorrhization to improve nutrition and growth of Jacaranda mimosifolia D.Don grown under abiotic constraints of the urban environment of Tunis city and to test the capacity of this kind of symbiose to substitute chemical fertilizers applications. The experiment was conducted using plot at the National Agronomic Institute of Tunisia located in Tunis. Plants about 1m in height are treated with indigenous mycorrhizal strains complex and a commercial product equivalent of 1kg / plant and 25 g / plant rates. For each inoculum, three fertilizer rates (15+ 9+ 12 (+2.5)) are tested using 0g, 2g and 4g corresponding to the producer recommended rate (0%, 50% and 100%). Results indicated that the infectious potential of indigenous strains is higher than strains contained within the commercial inoculum. Mycorrhization rates of the plants inoculated with indigenous mycorrhizal strains complex and those with commercial product are respectively of 43.82% and 20.86%. The combined fertilizer application and inoculation has no effect on mycorrhizal strains infectivity. Mycorrhization improves nitrogen, phosphorus, potassium and calcium woody nutrition (p = 0.01). Improvements are observed in the ability to acquire iron, magnesium and manganese by some treatments of mycorrhized Jacaranda. No effect of mycorrhization is found in terms of its zinc and sodium absorption. Jacaranda mycorrhization improves its mineral nutrition more effectively than fertilizer application adapted to the requirements of the plant. It improves its growth by increasing heights and stems and leaves dry weights.


Keywords: Arbuscular mycorrhizal fungi; mineral nutrition; growth; urban conditions; chemical fertilization.


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Mineral and phenolic content of leaves of Tunisian caprifig (Ficus carica L.) accessions

A. Essid1*

F. Aljane1

A. Ferchichi1, 2


1 Laboratoire d’Aridoculture et Cultures Oasiennes Institut des Régions Arides de Médenine, Médenine 4119, Tunisie

2 Institut National Agronomique de Tunisie, 43 Avenue Charles Nicolle, 1082 Cité Mahrajène, Tunis


Abstract: Leaves of twenty accessions of Tunisian caprifig (Ficus carica L.) accessions were investigated for their mineral and phenolic content. Results showed significant differences among accessions for all traits studied. Furthermore, leaves of caprifig are an important source of phenolic and mineral content. Potassium (379.05-1412.84mg/100g) is the major mineral content, followed by calcium (499.20mg/100g), magnesium (320.06mg/100g), phosphorus (175.54mg/100g), sodium (160.36mg/100g), iron (39.08mg/100g), manganese (2.54mg/100g) and zinc (1.41mg/100g). Total phenolic, flavonoid and flavonol contents ranged between 11.88-36.13mg.g-1GAE DW; 5.88-20 mg.g-1QE DW; 3-11.08 mg.g-1QE DW respectively. Cluster analysis and PCA grouped accessions studied in four groups.


Keywords: Caprifig, Ficus carica L., leaves, mineral content, phenolic compound Tunisia.


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Salicylic acid-induced resistance against Fusarium oxysporumf.s.pradicis lycopercisi in hydroponic grown tomato plants

W. Jendoubi*1

K. Harbaoui2

W. Hamada3,4


1 Warda Jendoubi, National institute of agricultural research, laboratory of field crops,Av Hédi Karray 2080 Ariana, Tunis, Tunisia,

2 Kalthoum, Harbaoui, Regional Field Crops Research Center (CRRGC), Béja-Tunisia Address: B. P. 350 Béja 9000, Tunisia, 

3 Walid Hamada, National Agronomic Institute of Tunisia, Laboratory of Genetic and Plant Breeding, 43 Av Charles Nicolle, 1082 Tunis, Tunisia.

4 Higher Agriculture School of Kef, Tunisia


Abstract - Salicylic acid (SA) is a plant hormone that plays an important role in induction of plant defense against a variety of biotic and abiotic stresses through morphological, physiological and biochemical mechanisms. In our study we have observed that plants grown in presence of SA show healthy appearance (Fusarium wilt symptoms) and an optimal vegetative growth compared to inoculated plants. The aimed objective of this work is to deduce if an exogenous application of salicylic acid can induce the accumulation of soluble phenolic compound in leaves of hydroponic tomato plants. This result is confirmed 24h after treatment when a gradual increase in peroxidase activity 24h after SA roots feeding were observed in roots and leaves. RT-PCR expression analysis of the gene encoding phenyl-amenia-lyase (PAL) and peroxidase (PR9) confirmed those results. The present work also showed that the PR-proteins such as glucanase (PR4), and chitinase (PR2) quickly responded to salicylic treatment and a high accumulation level of PR4 and PR2 transcripts was detected earlier at 48h and 72h post treatment through root feeding. These results suggest that SA at 200µM is safe to tomato plants and could be recommended for the stimulation of Fusarium defense which could be used biologic management programs.


Keywords: Fusarium oxysporum f.s.pradicis lycopercisi, gene expression, peroxidase, Salicylic Acid, soluble phenolic compounds, systemic acquired resistance


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This article is published under license to Journal of New Sciences. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

CC BY 4.0